CNR ISAFOM

Studio e analisi dei processi fisici, chimici e biologici che determinano il funzionamento e la dinamica degli agro-ecosistemi negli ambienti a clima mediterraneo, per il miglioramento quali-quantitativo delle produzioni, per l’ottimizzazione dell’uso delle risorse naturali, e per la valorizzazione delle funzioni dei sistemi agricoli e forestali. Sviluppo e applicazione di metodi di ricerca e di innovazioni tecnologiche avanzate per il monitoraggio e la previsione degli impatti e delle risposte degli ecosistemi agrari e forestali alle pressioni antropiche e climatiche.

L’ISAFOM afferisce al Dipartimento di Scienze Bio-Agroalimentari del CNR ed ha una "massa critica" di 89 unità ripartito tra le sedi di Portici - NA (sede centrale), Catania, Rende (CS), e Perugia.

Biosystems EngineeringVolume 200, December 2020, Pages 112-122

 

The relationship between seed physical characteristics and seed quality is widely investigated by using X-ray based imaging techniques. Recently the use of X-ray micro-tomography (micro-CT) is increasingly used for more accurate characterisation of the internal seed morphology.

In this work a germination test was carried out along with the morphometric characterisation of tomato seed internal structure by means of X-ray micro-CT and 3D image analysis. The aim was to accurately evaluate the predictive potential of internal seed 3D morphology for germination outcomes. The visual assessment allowed the relationship between specific internal seed abnormalities and the different germination outcomes to be demonstrated experimentally. Univariate analysis of morphometric seed traits allowed 3D free space % and Sauter diameter, among the most discriminant parameters, to be identified as the most effective for the prediction of germination outcomes. Discriminant Analysis (DA) of 3D morphometric dataset correctly classified 96.3% of normal seedlings, 83.3% of ungerminated seeds and 63.6% of abnormal seedlings, providing a high overall prediction potential of 91.9%. The above analyses have also been performed referring to the germination at 5 days after sowing. As a side effect of the applied technique, an increase of abnormal seedlings was observed at increasing X-ray exposure level. Overall, X-ray micro-CT coupled with DA of internal morphometric traits has proved to be an effective tool to investigate the relationship between tomato seed 3D morphology and seed physiology, although attention has to be paid to possible consequences of X-ray exposure.

By Laura GargiuloCristina LeonarduzziGiacomo Mele

LINK https://www.sciencedirect.com/science/article/pii/S1537511020302440?via%3Dihub


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